High throughput mass
cytometry - Doublet Recovery

Resolves overlapping events by analyzing the full ion cloud, enabling up to 3× higher acquisition speed without compromising resolution

Doublet Recovery™

Unlock high-throughput mass cytometry — without losing single-cell fidelity

Mass cytometry is powerful, but throughput is a bottleneck. Most instruments are capped at ~300 events/second to avoid doublets — multiple cells detected as a single event — which contaminate your data.

Doublet Recovery™
changes that

By analyzing the full ion cloud shape of each event, Doublet Recovery™ identifies and splits doublets, triplets, and even quadruplets into their original singlets. This allows you to:

• Increase throughput 3× — run up to 700+ clean single-cell events/second
• Recover rare samples — even from existing datasets, post-acquisition
• Expand daily capacity — go from 2–3 runs/day to 6–8, with no extra instrument time

Whether you're running large batch experiments or rescuing precious clinical material, Doublet Recovery™ helps you get more from every run — faster, cleaner, and with higher confidence.

How it works

01 Multiplet detection at raw level

Pre-FCS algorithm detects multiplets at the raw data level

02 Singlet reconstruction

Singlet reconstruction splits each event into its original components

03 Download cloud-native result

Enhanced FCS files with all recovered events are returned for analysis

Proven across sample densities up to 1.3M cells/mL with >90% recovery rate, Doublet Recovery™ pushes your instrument’s limits — not your data’s quality.

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Trusted by researchers worldwide to bring
consistency and depth to immune data.

We use SCIDENTIFY tech to develop internal standards for our state-of-the-art optical process. Their ability to access information in the ion cloud is unprecedented.

Prof. Dr. Sara Skrabalak
Director CSENND, Indiana University, Bloomington | Editor-in-Chief, Chemistry of Materials

SCIDENTIFYs morphological parameters enhance the accuracy of downstream clustering algorithms.

Dr. Christoph SCHwärzler
Mass cytometry specialist, EPFL | Ex-Novartis

We use SCIDENTIFY services to improve our fluorescent nanoparticle product-line. They have provided us such a deep-understanding of our system. Amazing!

Lars dähne
CEO, Surflay Nanotec GmbH